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KMID : 0376119940210020273
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Medical Journal of the Red Cross Hospital
1994 Volume.21 No. 2 p.273 ~ p.282
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The Effects of a Single Exposure of UVB on Morphologic Changes and Proliferation in Cultured Normal Melanocytes
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Abstract
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in humans, the major stimulus for cutaneous pigmentation is ultraviolet radiation. Little is known about the mechanism underlying this response, in part because of the complexity of the interactions involving the whole epidermis. The present
study
was
undertaken to evaluate the effects of a single exposure of UVB on cultured normal melanocytes. Melanocytes were exposed to UVB from 50.8mJ/㎠ to 203mJ/㎠
@ES the results were as follows:
@EN 1) The main morphologic changes in UVB-exposed groups were larger sized cells, more blunted dendrites, and shorter dendrites than in the control group. These cells increased sized according to the increased doses of UVB, but above 101.5mJ/㎠.
The
melanocytes shrunk and were destroyed.
2) From20.3mJ/㎠ of UVB, the proliferation of melanocytes was decreased. Especially, there was statistically significant difference above 50.8mJ/㎠(P<0.005, P<0.01).
3) The antiproliferative effect increased with the passage of time after UVB exposure. So cell count could not be done in 101.5mJ/㎠ and 203mJ/㎠ on third day, and in 50.8mJ/㎠, 101.5mJ/㎠ and 203mJ/㎠ on seventh day.
4) Statistically the melanin content per well was significantly decreased to 11~28% of each control group with dose above 50.8mJ/㎠(p<0.05, P<0.01).
The melanin content per cell was increased to 107~128% of each control group when doses were below 20.3mJ/㎠ and decreased to 49~79% of each control group when above 50.8mJ/㎠ on third day, but there was no statistically significant difference.
In summary, when melanocytes were exposed to UVB, morphologic changes progressed to cell differentiation. The results also suggested that a lower dose of UVB has an antiproliferative and mild melanogenic effect, and a higher dose of UVB has a
direct
cytotoxic effect.
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